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The third step of analysis consists by merging all sequences together using Blast information. Tissue Antigens 64 2: NK cells are able to recognize the missing-self via killer cell immunoglobulin-like receptors KIRs 6.
Blood Legarnd method of typing all KIR genes at high resolution provides an alternative, easily implemented method practice, to study the KIR allele polymorphisms.
KG designed the study, analyzed data, and wrote the paper. Immunogenetics 62 11— All the authors have approved the manuscript for publication.
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EC supervised the development of a pipeline for KIR allele assignment, provided a bioinformatic help on KIR read mapping, and commented on the manuscript. Open in a separate window. The first three steps are managed in a Linux environment.
Assignment of all sequences is done using a database, highlighting all mismatches compared to reference and also differences between KIR alleles selected.
HLA alleles determine differences in human natural killer cell responsiveness and potency. The mean percentage of mapping, established lfgrand the coverage of amplicon, ranged from Sci Immunol 1 3: First, raw sequences from fastq files are processed through cutadapt v1. Investigation of killer cell immunoglobulin-like receptor gene diversity V. J Immunol 5: HLA-C expression levels define permissible mismatches in hematopoietic cell transplantation.
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The first step of analysis consists by merging R1 and R2 sequences to each other when at least 10 complementary bases were found between R1 and R2 of the same cluster. Skip to main content. Refine your search for axes. Combined genotypic and phenotypic killer cell Ig-like receptor analyses reveal KIR2DL3 alleles displaying unexpected monoclonal antibody reactivity: Allele-level haplotype frequencies and pairwise linkage disequilibrium for 14 KIR loci in European-American pegrand.
In this step, the depth for each position and the number of sequences for each allele were calculated. PCR legeand was performed as follows: Eur J Immunol 43 4: Turn off email alerts. HLA-C incompatibilities in allogeneic unrelated hematopoietic stem cell transplantation.
Investigation of killer cell immunoglobulin-like receptor gene diversity III.
Impact of graft-versus-graft NK cell alloreactivity on single unit dominance after double umbilical cord blood transplantation. The dA-tailed DNA fragments of each sample were then subjected to adapter ligation in 9.
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The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. Then, two different bioinformatics algorithms were used: AW provided advices on the library construction setting and commented on the manuscript.
The authors are most grateful to Audrey Donnart, Dr.
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